T7941
 |  |  |
 | Immunoblotting MCF7 Human breast carcinoma cells extract was separated on SDS-PAGE and probed with Monoclonal AB To β Tubulin IV CL:ONS.1A6 (Cat. No. T7941). The antibody was developed with AlkPhos APA Mouse Fab ads HIgG (Cat. No. A1293) and a NBT/BCIP substrate. Antibody dilution 1/400. |
Image
Useful Links & Tools
- MSDS
- Certificate of Analysis
- Bulk Quote
- Similar Products
T7941
Sigma
Monoclonal Anti-β-Tubulin IV antibody produced in mouse
clone ONS.1A6, ascites fluid
| MDL number: | MFCD02095891 |
Description
| Immunogen | synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype IV, conjugated to BSA. |
| Specificity | Recognizes an epitope located in the C-terminal sequence of β-tubulin isotype IV. No reactivity with other tubulin isotypes is observed. |
Properties
| antibody form | ascites fluid |
| clone | ONS.1A6 (monoclonal) |
| mol wt | antigen mol wt ~55 kDa |
| species reactivity | human, hamster, chicken, bovine, rat, mouse |
| application(s) | immunoblotting: 1:400 using cultured human breast adenocarcinoma cell line MCF-7 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| isotype | IgG1 |
| Gene Information | human ... TUBB4(10382) |
| rat ... Tubb4(29213) | |
| mouse ... Tubb4(22153) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
References
| reference | Banerjee, A., et al. J. Biol. Chem. 267, 5625, (1992) Abstract |
| Roach, M.C., et al. Cell Motil. Cytoskeleton 39, 273, (1998) Abstract | |
| Renthal, R., et al. Cell Motil. Cytoskeleton 25, 19, (1993) Abstract | |
| Banerjee, A. and Luduena, R.F. J. Biol. Chem. 267, 13335, (1992) Abstract | |
| Sharma, J. and Luduena, R.F. J. Protein Chem. 13, 165, (1994) Abstract | |
| Khan, I.A. and Luduena, R.F. Biochemistry 35, 3704, (1996) Abstract | |
| Fujimoto, M., et al., Heat shock transcription factor 1 is required for maintenance of ciliary beating in mice. J. Biol. Chem. 282, 37285-37292, (2007) Abstract | |
| Banerjee, A., et al., Direct involvement of the isotype-specific C-terminus of β-tubulin in ciliary beating J. Cell Sci. 118, 4333-4341, (2005) Abstract | |
| Cynamon, M., et al., Activity of n-propyl pyrazinoate against pyrazinamide-resistant Mycobacterium tuberculosis: investigations into mechanism of action of and mechanism of resistance to pyrazinamide. Antimicrob. Agents Chemother. 39, 1269-1271, (1995) Abstract |
